Hello,
I am Subash

• Proficient in data analysis, data interpretation and scientific communication as demonstrated by published papers, research seminar presentations, grant writing.
• Designed and executed drug efficacy studies utilizing mice models of Alzheimer’s disease. Mice models of AD studied: 3xTg AD, App23, Tg2576.
• Performed of Bilateral typical carotid artery Occlusion (BCAO) surgery in rodents’ model of stroke.
• Performed different rodent behavior tests in mice to assess the efficacy of drug candidates. Behavioral test utilized: Morris water maze (Spatial reference memory, Working memory, memory extinction and cognitive flexibility), Force plate actometer, open field test (Locomotor activity, Behavioral and psychological symptoms of dementia), Rotarod (motor coordination and strength), Radial maze (working memory).
• Extensive experience performing trans-cardiac perfusion in mice using paraformaldehyde followed by cryostat sectioning for Immunohistochemistry and immunofluorescence.
• Performed and excelled at multiple biochemical tests such as Western blot, immunohistochemistry, immunofluorescence, PCR, and RT-qPCR. Skills acquired include the ability to troubleshoot, design probes and primers, select and troubleshoot antibodies and their expression, and analyze and interpret data.
• Proficiency in acquiring images, analyzing data, and trouble systems such as the Nikon N-SIM super-resolution system with A1+confocal on the Eclipse Ti2 microscope, Nikon A1R multiphoton with FN1 Upright microscope, and the Zeiss LSM 700 confocal system. Proficient with image analysis systems such as NIH ImageJ-FIJI, NIS-element with advanced research 5.0 and deconvolution module, and Amira 3D image reconstruction software.
• Proficient in data analysis using GraphPad Prism, Image Studio, R programming, and Python (Basic)-ongoing)  
• Expensive experience in neuronal culture and differentiation protocols using multiple cell lines, including PC-12, SH-SY5Y, astrocytes, and microglia cultures. Proficient in cell viability assay such as ATP and MTT and cellular toxicity assay such as LDH assay.
• Extensive experience with laboratory management and supervision including inventory management of lab animals (mice), chemical inventory management, management of lab personals, and ordering lab reagents, among other things
• Experience in engagement with laboratory regulatory agencies (IUCAC, IRB, Environmental Health & Safety, and the research integrity offices) for lab inspections and removal/improvement of deficiencies.

Mentorship and training

• Trained and mentored 15+ new and existing research associates (prospective Ph.D., graduate, undergraduate, and High School students, and research volunteers) on laboratory techniques such as animal surgeries, animal behavior tests, biochemical tests, as wells as data analysis and interpretations.
• Mentored 100+ Graduate teaching assistants in teaching undergraduate students in biology Laboratory classes, including leading weekly teaching team meetings, demonstrating teaching methods, and demonstrating laboratory techniques.